Gene Trap Vectors
This page displays descriptions of the various gene trapping vectors1 that are used in the EUCOMM pipeline.
rsFlipROSAβgeo(Cre)∗
| Description | This retroviral gene-trap vector uses Flpe recombinase for the first inversion of the gene-trap cassette using the unidirectional FIEx system (frt and F3). For the second inversion a FIEx configurated Cre recombinase system is used (loxP and lox5171). Furthermore the spacer between the 5'frt site and the F3 site has been substituted. To enhance the sensitivity of the neomycin cassette the neomycin resistance gene was modified. This vector is used in frameshift variations. Additional nucleotides were introduced into the adenoviral splice acceptor. |
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| Sequence of Reading Frames |
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| Provirus Sequence | TGAAAGACCC CCGCTGACGG GTAGTCAATC ACTCAGAGGA GACCCTCCCA AGGAACAGCG AGACCACAAG TCGGATGCAA CTGCAAGAGG GTTTATTGGA TACACGGGTA CCCGGGCGAC TCAGTCAATC GGAGGACTGG CGCCCCGAGT GAGGGGTTGT GCTAGCTTGC CAAACCTACA GGTGGGGTCT TTCATTCCCC CCTTTTTCTG GAGACTAAAT AAAATCTTTT ATTTTATCGA TTCAAGCCAC TATGCGCACA GCTGGTCGAG CCCGGGCCCT CGAGAATTCG AAGTTCCTAT TCCGAAGTTC CTATTCTCTA GAAAGTATAG GAACTTCAGC AGATCCTTAT TTGTCATATT GTAAGTGCTT TCTCTAATCA CTTTCTTTCA TGGCAAGCAG GGTATGGATC TGCTGAAGTT CCTATTCCGA AGTTCCTATT CTTCAAATAG TATAGGAACT TCGTTGCTAG AAGCGGTTTT CGGGAGAATA CGACTCACTA TAGGGCGAAT TGATAACTTC GTATAGCATA CATTATACGA AGTTATCCAA GCTTCACCAT CGACCCGAAT TGCCAAGCAT CACCATCGAC CCATAACTTC GTATAGTACA CATTATACGA AGTTATCGAA TTCCTACTCG AGGCTAGAAC TAGTGGATCC CCCGGGCTGC AGATCTGTAG GGCGCAGTAG TCCAGGGTTT CCTTGATGAT GTCATACTTA TCCTGTCCCT TTTTTTTCCA CAGCTCGCGG TGAGGACAAA CTCTTCGCGG TCTTTCCAGT GGGGATCGAC GGTATCGATA AGCTTGATGA TCTGTGACAT GGCGGATCCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCTTTGCCT GGTTTCCGGC ACCAGAAGCG GTGCCGGAAA GCTGGCTGGA GTGCGATCTT CCTGAGGCCG ATACTGTCGT CGTCCCCTCA AACTGGCAGA TGCACGGTTA CGATGCGCCC ATCTACACCA ACGTGACCTA TCCCATTACG GTCAATCCGC CGTTTGTTCC CACGGAGAAT CCGACGGGTT GTTACTCGCT CACATTTAAT GTTGATGAAA GCTGGCTACA GGAAGGCCAG ACGCGAATTA TTTTTGATGG CGTTAACTCG GCGTTTCATC TGTGGTGCAA CGGGCGCTGG GTCGGTTACG GCCAGGACAG TCGTTTGCCG TCTGAATTTG ACCTGAGCGC ATTTTTACGC GCCGGAGAAA ACCGCCTCGC GGTGATGGTG CTGCGTTGGA GTGACGGCAG TTATCTGGAA GATCAGGATA TGTGGCGGAT GAGCGGCATT TTCCGTGACG TCTCGTTGCT GCATAAACCG ACTACACAAA TCAGCGATTT CCATGTTGCC ACTCGCTTTA ATGATGATTT CAGCCGCGCT GTACTGGAGG CTGAAGTTCA GATGTGCGGC GAGTTGCGTG ACTACCTACG GGTAACAGTT TCTTTATGGC AGGGTGAAAC GCAGGTCGCC AGCGGCACCG CGCCTTTCGG CGGTGAAATT ATCGATGAGC GTGGTGGTTA TGCCGATCGC GTCACACTAC GTCTGAACGT CGAAAACCCG AAACTGTGGA GCGCCGAAAT CCCGAATCTC TATCGTGCGG TGGTTGAACT GCACACCGCC GACGGCACGC TGATTGAAGC AGAAGCCTGC GATGTCGGTT TCCGCGAGGT GCGGATTGAA AATGGTCTGC TGCTGCTGAA CGGCAAGCCG TTGCTGATTC GAGGCGTTAA CCGTCACGAG CATCATCCTC TGCATGGTCA GGTCATGGAT GAGCAGACGA TGGTGCAGGA TATCCTGCTG ATGAAGCAGA ACAACTTTAA CGCCGTGCGC TGTTCGCATT ATCCGAACCA TCCGCTGTGG TACACGCTGT GCGACCGCTA CGGCCTGTAT GTGGTGGATG AAGCCAATAT TGAAACCCAC GGCATGGTGC CAATGAATCG TCTGACCGAT GATCCGCGCT GGCTACCGGC GATGAGCGAA CGCGTAACGC GAATGGTGCA GCGCGATCGT AATCACCCGA GTGTGATCAT CTGGTCGCTG GGGAATGAAT CAGGCCACGG CGCTAATCAC GACGCGCTGT ATCGCTGGAT CAAATCTGTC GATCCTTCCC GCCCGGTGCA GTATGAAGGC GGCGGAGCCG ACACCACGGC CACCGATATT ATTTGCCCGA TGTACGCGCG CGTGGATGAA GACCAGCCCT TCCCGGCTGT GCCGAAATGG TCCATCAAAA AATGGCTTTC GCTACCTGGA GAGACGCGCC CGCTGATCCT TTGCGAATAC GCCCACGCGA TGGGTAACAG TCTTGGCGGT TTCGCTAAAT ACTGGCAGGC GTTTCGTCAG TATCCCCGTT TACAGGGCGG CTTCGTCTGG GACTGGGTGG ATCAGTCGCT GATTAAATAT GATGAAAACG GCAACCCGTG GTCGGCTTAC GGCGGTGATT TTGGCGATAC GCCGAACGAT CGCCAGTTCT GTATGAACGG TCTGGTCTTT GCCGACCGCA CGCCGCATCC AGCGCTGACG GAAGCAAAAC ACCAGCAGCA GTTTTTCCAG TTCCGTTTAT CCGGGCAAAC CATCGAAGTG ACCAGCGAAT ACCTGTTCCG TCATAGCGAT AACGAGCTCC TGCACTGGAT GGTGGCGCTG GATGGTAAGC CGCTGGCAAG CGGTGAAGTG CCTCTGGATG TCGCTCCACA AGGTAAACAG TTGATTGAAC TGCCTGAACT ACCGCAGCCG GAGAGCGCCG GGCAACTCTG GCTCACAGTA CGCGTAGTGC AACCGAACGC GACCGCATGG TCAGAAGCCG GGCACATCAG CGCCTGGCAG CAGTGGCGTC TGGCGGAAAA CCTCAGTGTG ACGCTCCCCG CCGCGTCCCA CGCCATCCCG CATCTGACCA CCAGCGAAAT GGATTTTTGC ATCGAGCTGG GTAATAAGCG TTGGCAATTT AACCGCCAGT CAGGCTTTCT TTCACAGATG TGGATTGGCG ATAAAAAACA ACTGCTGACG CCGCTGCGCG ATCAGTTCAC CCGTGCACCG CTGGATAACG ACATTGGCGT AAGTGAAGCG ACCCGCATTG ACCCTAACGC CTGGGTCGAA CGCTGGAAGG CGGCGGGCCA TTACCAGGCC GAAGCAGCGT TGTTGCAGTG CACGGCAGAT ACACTTGCTG ATGCGGTGCT GATTACGACC GCTCACGCGT GGCAGCATCA GGGGAAAACC TTATTTATCA GCCGGAAAAC CTACCGGATT GATGGTAGTG GTCAAATGGC GATTACCGTT GATGTTGAAG TGGCGAGCGA TACACCGCAT CCGGCGCGGA TTGGCCTGAA CTGCCAGCTG GCGCAGGTAG CAGAGCGGGT AAACTGGCTC GGATTAGGGC CGCAAGAAAA CTATCCCGAC CGCCTTACTG CCGCCTGTTT TGACCGCTGG GATCTGCCAT TGTCAGACAT GTATACCCCG TACGTCTTCC CGAGCGAAAA CGGTCTGCGC TGCGGGACGC GCGAATTGAA TTATGGCCCA CACCAGTGGC GCGGCGACTT CCAGTTCAAC ATCAGCCGCT ACAGTCAACA GCAACTGATG GAAACCAGCC ATCGCCATCT GCTGCACGCG GAAGAAGGCA CATGGCTGAA TATCGACGGT TTCCATATGG GGATTGGTGG CGACGACTCC TGGAGCCCGT CAGTATCGGC GGAATTCCAG CTGAGCGCCG GTCGCTACCA TTACCAGTTG GTCTGGTGTC AGGGGATCCC CCGGGCTGCA GCCAATATGG GATCGGCCAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GGGGATCAAT TCTCTAGAGC TCGCTGATCA GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG ACGGAAAGAA CCAGCTGGGG CTCGATCCTC TAGAGTCGAC CTCGAGTACC ACCACACTGG GATCCGATAA CTTCGTATAA TGTATGCTAT ACGAAGTTAT CCAAGCATCA CCATCGACCC TCTAGTCCAG ATCTCACCAT CGACCCATAA CTTCGTATAA TGTGTACTAT ACGAAGTTAT TCTAGACTCT TCCGCTTCCT CGCTCCACCG CGGCTTCGAG ACCGTCACGA GAAGTTCCTA TACTTTCTAG AGAATAGGAA CTTCGGAATA GGAACTTCGT TAACGAAGTT CCTATACTAT TTGAAGAATA GGAACTTCGG AATAGGAACT TCAGCAACGG ATCCGGCCGG CGCCTAGAGA AGGAGTGAGG GCTGGATAAA GGGAGGATCG AGGCGGGGTC GAACGAGGAG GTTCAAGGGG GAGAGACGGG GCGGATGGAG GAAGAGGAGG CGGAGGCTTA GGGTGTACAA AGGGCTTGAC CCAGGGAGGG GGGTCAAAAG CCAAGGCTTC CCAGGTCACG ATGTAGGGGA CCTGGTCTGG GTGTCCATGC GGGCCAGGTG AAAAGACCTT GATCTTAACC TGGGTGATGA GGTCTCGGTT AAAGGTGCCG TCTCGCGGCC ATCCGACGTT AAAGGTTGGC CATTCTGCAG AGCAGAAGGT AACCCAACGT CTCTTCTTGA CATCTACCGA CTGGTTGTGA GCGATCCGCT CGACATCTTT CCAGTGACCT AAGGTCAAAC TTAAGGGAGT GGTAACAGTC TGGCCCTAAT TCTCAGACAA ATACAGAAAC ACAGTCAGAC AGAGACAACA CAGAACGATG CTGCAGCAGA CAAGACGCGC GGCGCGGCTT CGGTCCCAAA CCGAAAGCAA AAATTCAGAC GGAGGCGGGA ACTGTTTTAG GTTCTCGTCT CCTACCAGAA CCACATATCC CTCCTCTAAG GGGGGTGCAC CAAAGAGTCC AAAACGATCG GGATTTTTGG ACTCAGGTCG GGCCACAAAA ACGGCCCCCG AAGTCCCTGG GACGTCTCCC AGGGTTGCGG CCGGGTGTTC CGAACTCGTC AGTTCCACCA CGGGTCCGCC AGATACAGAG CTAGTTAGCT AACTAGTACC GACGCAGGCG CATAAAATCA GTCATAGACA CTAGACAATC GGACAGACAC AGATAAGTTG CTGGCCAGCT TGCCTCCCGG TGGTGGGTCG GTGGTCCCTG GGCAGGGGTC TCCCGATCCC GGACGAGCCC CCAAATGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG GTTGTGCTAG CTTGCCAAAC CTACAGGTGG GGTCTTTCAT TCCCCCCTTT TTCTGGAGAC TAAATAAAAT CTTTTATTTT ATCGAT |
rsFlipROSACeo-2
| Description | This retroviral gene-trap vector predominantly integrates into genes of the secretory pathways. It contains a Ceo-pA cassette with a cryptic Splice Acceptor sequence of the human CD2 domain. Ceo is an in-frame fusion between the human CD2 cell surface receptor and the neomycin-resistance gene. |
| Sequence of Reading frame | CAAAGAGATTGAATGCCTTG |
| Provirus Sequence | TGAAAGACCC CCGCTGACGG GTAGTCAATC ACTCAGAGGA GACCCTCCCA AGGAACAGCG AGACCACAAG TCGGATGCAA CTGCAAGAGG GTTTATTGGA TACACGGGTA CCCGGGCGAC TCAGTCAATC GGAGGACTGG CGCCCCGAGT GAGGGGTTGT GCTAGCTTGC CAAACCTACA GGTGGGGTCT TTCATTCCCC CCTTTTTCTG GAGACTAAAT AAAATCTTTT ATTTTATCGA TTCAAGCCAC TATGCGCACA GCTGGTCGAG CCCGGGCCCT CGAGAATTCG AAGTTCCTAT TCCGAAGTTC CTATTCTCTA GAAAGTATAG GAACTTCAGC AGATCCTTAT TTGTCATATT GTAAGTGCTT TCTCTAATCA CTTTCTTTCA TGGCAAGCAG GGTATGGATC TGCTGAAGTT CCTATTCCGA AGTTCCTATT CTTCAAATAG TATAGGAACT TCGTTGCTAG AAGCGGTTTT CGGGAGAATA CGACTCACTA TAGGGCGAAT TGATAACTTC GTATAGCATA CATTATACGA AGTTATCCAA GCTTCACCAT CGACCCGAAT TGCCAAGCAT CACCATCGAC CCATAACTTC GTATAGTACA CATTATACGA AGTTATCGAA TTCCTACGTA CTAGATTCCA TGTAAATTTG TAGCCAGCTT CCTTCTGATT TTCAATGTTT CTTCCAAAGG TGCAGTCTCC AAAGAGATTG AATGCCTTGG AAACCTGGGG TGCCTTGGGT CAGGACATCA ACTTGGACAT TCCTAGTTTT CAAATGAGTG ATGATATTGA CGATATAAAA TGGGAAAAAA CTTCAGACAA GAAAAAGATT GCACAATTCA GAAAAGAGAA AGAGACTTTC AAGGAAAAAG ATACATATAA GCTATTTAAA AATGGAACTC TGAAAATTAA GCATCTGAAG ACCGATGATC AGGATATCTA CAAGGTATCA ATATATGATA CAAAAGGAAA AAATGTGTTG GAAAAAATAT TTGATTTGAA GATTCAAGAG AGGGTCTCAA AACCAAAGAT CTCCTGGACT TGTATCAACA CAACCCTGAC CTGTGAGGTA ATGAATGGAA CTGACCCCGA ATTAAACCTG TATCAAGATG GGAAACATCT AAAACTTTCT CAGAGGGTCA TCACACACAA GTGGACCACC AGCCTGAGTG CAAAATTCAA GTGCACAGCA GGGAACAAAG TCAGCAAGGA ATCCAGTGTC GAGCCTGTCA GCTGTCCAGA GAAAGGTCTG GACATCTATC TCATCATTGG CATATGTGGA GGAGGCAGCC TCTTGATGGT CTTTGTGGCA CTGCTCGTTT TCTATATCAC CAAAAGGAAA AAACAGAGGA GTCGGAGAAA TGATGAGGAG CTGGAGACAA GAGCCCACAG AGTAGCTACG GATCCCATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGG GGATCAATTC TCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG CTTCTGAGAC GGAAAGAACC AGCTGGGGCT CGATCCTCTA GAGTCGACCT CGAGTACCAC CACACTGGGA TCCGATAACT TCGTATAATG TATGCTATAC GAAGTTATCC AAGCATCACC ATCGACCCTC TAGTCCAGAT CTCACCATCG ACCCATAACT TCGTATAATG TGTACTATAC GAAGTTATTC TAGACTCTTC CGCTTCCTCG CTCCACCGCG GCTTCGAGAC CGTCACGAGA AGTTCCTATA CTTTCTAGAG AATAGGAACT TCGGAATAGG AACTTCGTTA ACGAAGTTCC TATACTATTT GAAGAATAGG AACTTCGGAA TAGGAACTTC AGCAACGGAT CCGGCCGGCG CCTAGAGAAG GAGTGAGGGC TGGATAAAGG GAGGATCGAG GCGGGGTCGA ACGAGGAGGT TCAAGGGGGA GAGACGGGGC GGATGGAGGA AGAGGAGGCG GAGGCTTAGG GTGTACAAAG GGCTTGACCC AGGGAGGGGG GTCAAAAGCC AAGGCTTCCC AGGTCACGAT GTAGGGGACC TGGTCTGGGT GTCCATGCGG GCCAGGTGAA AAGACCTTGA TCTTAACCTG GGTGATGAGG TCTCGGTTAA AGGTGCCGTC TCGCGGCCAT CCGACGTTAA AGGTTGGCCA TTCTGCAGAG CAGAAGGTAA CCCAACGTCT CTTCTTGACA TCTACCGACT GGTTGTGAGC GATCCGCTCG ACATCTTTCC AGTGACCTAA GGTCAAACTT AAGGGAGTGG TAACAGTCTG GCCCTAATTC TCAGACAAAT ACAGAAACAC AGTCAGACAG AGACAACACA GAACGATGCT GCAGCAGACA AGACGCGCGG CGCGGCTTCG GTCCCAAACC GAAAGCAAAA ATTCAGACGG AGGCGGGAAC TGTTTTAGGT TCTCGTCTCC TACCAGAACC ACATATCCCT CCTCTAAGGG GGGTGCACCA AAGAGTCCAA AACGATCGGG ATTTTTGGAC TCAGGTCGGG CCACAAAAAC GGCCCCCGAA GTCCCTGGGA CGTCTCCCAG GGTTGCGGCC GGGTGTTCCG AACTCGTCAG TTCCACCACG GGTCCGCCAG ATACAGAGCT AGTTAGCTAA CTAGTACCGA CGCAGGCGCA TAAAATCAGT CATAGACACT AGACAATCGG ACAGACACAG ATAAGTTGCT GGCCAGCTTG CCTCCCGGTG GTGGGTCGGT GGTCCCTGGG CAGGGGTCTC CCGATCCCGG ACGAGCCCCC AAATGAAAGA CCCCCGCTGA CGGGTAGTCA ATCACTCAGA GGAGACCCTC CCAAGGAACA GCGAGACCAC AAGTCGGATG CAACTGCAAG AGGGTTTATT GGATACACGG GTACCCGGGC GACTCAGTCA ATCGGAGGAC TGGCGCCCCG AGTGAGGGGT TGTGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA AATAAAATCT TTTATTTTAT CGAT |