Gene Slc7a7: Alleles suitable for CRISPR-assisted targeting
The regions replaced by the 3' loxP site in the targeting vectors are highlighted in a short snippet of the wildtype sequence.
To get more information about how to use our targeting vectors with CRISPR technologies, please refer to our publication.
Allele #25885
| Mutation Type: | Knockout-first Allele (with Conditional Potential) / Frameshift |
| Cassette: | L1L2_Bact_P / promotor driven |
| Backbone: | L3L4_pD223_DTA_T_spec |
| Targeting Vectors: | Genbank File | Image |
| Assembly/Sequence Origin: | GRCm83.p4 |
| Chromosome: | 14 |
| Strand: | - |
| Deletion Size: | 132 |
| Deleted Region: | 54377478-54377609 |
Wildtype Sequence: (download as GenBank file)
12 possible gRNAs found: (hover to highlight)
Please note: There is no prioritization on the order of the sequences. They are just displayed from left (5') to right (3'), forward strand first.
GCTTCACCCTCTTGAGTAGC +
ACCCTCTTGAGTAGCTGGCA +
GTAGCTGGCAAGGACCTTGC +
AGGACCTTGCAGGTGTCTAG +
AGTATAACCTGCTTTATTTT +
CATGTACAAACCTTCCTAAA +
TCCTTGCCAGCTACTCAAGA -
GTCCTTGCCAGCTACTCAAG -
TATGCCTCTAGACACCTGCA -
ATTTCTACCTAAAATAAAGC -
CTTTATAAATCCTTTTAGGA -
TATGCTTTATAAATCCTTTT -
Please note: There is no prioritization on the order of the sequences. They are just displayed from left (5') to right (3'), forward strand first.